Abstract: RocketIO,which is high -speed dedicated transceiver embedded in Virtex -5 family FPGA of Xilinx corporation,acts as physical layer.Both designs are implemented based on RocketIO,the one is high-speed transceiver based on customized transfer protocol,the other is high-speed serial interface(HSSI) based on the same one.A brief analysis is made between designs,related application occasion is explained.Taking high-speed signal acquisition,for example,the high -speed serial interface,based on customized transfer protocol,is implemented though verifying and analysing data that catched by chipscope.According to the result,customized transfer protocol, based on RocketIO,is reliable and error bit rate less than 10^-13.