Abstract: Based on the multi-channel and high-speed digitization features of front-end readout circuits for biomedical imaging, a 16-channel pipeline digitization circuits is designed.The whole circuits consist of analog multiplexer, single end to differential signal conversion circuit, 8-bit 25-Msample/s pipeline ADC, and data output module.Analog-to-digital conversion and data output will be executed in the pipeline way.The circuit is designed in TSMC 0.18 μm mixed signal CMOS technology.Simulation results show that, pipeline ADC has-0.62/0.67 LSB DNL, -0.39/0.72 LSB INL, 45.99 dB SNR, and 6.03 bit ENOB.The designed circuits can accomplish the digitization and output of 16-channel analog signals within two adjacent timing windows, satisfying the performance requirements of the system.