Abstract: Tag collision is one of the key problem of RFID system, which directly affects the efficiency of the system. For tag collision problem, a new anti-collision algorithm based on adjustive multi-tree was proposed on the basic of studying on present anti-collision algorithm. In this paper, the basic ideas and processes of new anti-collision algorithm were introduced. Then, a new implementation scheme of new anti-collision algorithm based on FPGA was designed and the simulation to the function of the key modules were carried on. The simulation results show the superiority and feasibility of the new algorithm.