Abstract: A wireless sensor network acoustic source localization system based on TelosB is designed in this article. This system consists of a control node, information collection nodes and a base station. Abnormal sampling lead by wake-up delay of the information collection nodes was resolved as well as the data block of the serial port. In localization algorithm, the time delay estimation localization algorithm based on the classical generalized cross correlation function was adopted. At the same time, in order to eliminate the effects brought by the non-ideal characteristics such as device mismatch and synchronization related noise among different nodes, we pre-filtered the signal and the generalized cross correlation function. It is demonstrated in experiments that the sound localization can realize acoustic source localization in indoor environment. For a positioning area of 9 m², absolute error of over 70% localization results are less than 30 cm.