Abstract: With the development of Ethernet technology and integrated circuit technology, the speed and performance of Ethernet Physical Layer (PHY) chips have been significantly enhanced, and the circuit complexity has increased geometrically. It is difficult to fully verify its function in conventional Automatic Test Equipment (ATE) testing, so it is urgent to carry out corresponding test method research. An efficient ZYNQ MPSOC based method for functional testing of Ethernet PHY chip is proposed. A system-level test device is designed directly to the application layer, which reduces the direct interaction with the physical layer and effectively reduces the difficulty of test device and program development. The experimental results show that the ZYNQ MPSOC based method can be used to test the function of Ethernet PHY chip.