Abstract: Silicon interposer based integration methodology can achieve high-speed optoelectronic interconnection, but there are many signal integrity problems. In order to meet the demand of high-speed optoelectronic interconnection on silicon interposer, this paper proposes a twisting method to improve the signal-to-noise ratio. Base on the electromagnetic simulation tool, the high-speed differential interconnects are modeled and simulated. The simulation results show that the proposed twisted differential interconnect has significant performance improvement on crosstalk cancelling. This paper also proposes optimization methods for the differential wires by adjusting the size and twisting position, which can further improve the transmission performance. Base on the simulation results, the proposed method can be used to improve the design of high-speed interconnect of silicon interposer.